Substrate concentration and yeast catalase

Use this information as a check on the practical set-up. Use the permanent marker to label the remaining four cups from one to four. The oxygen produced in 30 seconds is collected over water.

In case you spill hydrogen peroxide, clean it up with a wet paper towel. Students may notice the heat if they put their hands on the conical flask. Many other factors affect the activity of enzymes as well.

Effect of Substrate Concentration on the Rate of Activity of Catalase

There may also be some experimental error which causes the inaccuracies. Use this information as a check on the practical set-up.

Exploring Enzymes

Without sensors available, an easy and very reliable method is to use graduated cylinders filled with H2O2. As the syringe plunger is very slowly depressed, uniform drops of the yeast-sodium alginate solution will form spheres as they come in contact with the CaCl2 solution and these will fall to the bottom of the beaker.

This is shown in the table with all the results I have gained Fig. It is not only the acid in your stomach that breaks down your food—many little molecules in your body, called enzymes, help with that too. Doing this could lead to being disqualified from all the subjects that you are taking.

However, there are some factors that I must take into consideration. What happens if the environmental conditions for the enzyme are changed. This solution should be mixed well and then drawn up into a syringe.

To prevent this I had to dry out the barrel and syringe before commencing the procedure.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

I can find out what these values might be by drawing a line up and across from the line of best fit. The time taken to fit into and leave the active site is the limiting factor in the rate of reaction.

Ask an Expert The Ask an Expert Forum is intended to be a place where students can go to find answers to science questions that they have been unable to find using other resources. Abstract Yeast contains an enzyme, called catalase, that acts as a catalyst for the reaction that breaks down hydrogen peroxide into oxygen and water (2H 2 O 2 2H 2 O + O 2).Safety note: oxygen is a highly reactive gas, adult supervision recommended for this project.

For your background research, be sure that you understand substrate, catalyst, reaction rate, catalase, enzyme saturation and. Abstract.

Effect of Substrate Concentration on the Rate of Activity of Catalase

1. A re-examination of the catalase–hydrogen peroxide reaction at high substrate concentrations, by using the quenched-flow technique, reveals a more complex kinetic behaviour than that previously reported.

I am using yeast catalase as opposed to catalase from apples, potatoes or liver because it is easier to get the desired amount of yeast catalase by simply measuring it off. To obtain catalase from a substance such as potato would involve crushing it and with that method you would never be sure of the concentration of the catalase.

Apr 30,  · I believe that as the concentration of the hydrogen peroxide (substrate) decreases, the rate of reaction will decrease as well. This is because as there are progressively fewer molecules of hydrogen peroxide there will be fewer collisions between the substrate and enzyme molecules (catalase in yeast), leading to a decrease in enzyme-substrate Reviews: 5.

If the substrate concentration is increased then the rate of a catalase reaction will also increase until it reaches the optimal concentration or saturation point and will remain constant.

This is because there will be more substrate molecules in a higher concentration therefore a higher frequency of collisions. Substrate concentration and yeast catalase Aim: To see how the substrate concentration in hydrogen peroxide affects the rate of an enzyme controlled reaction using yeast catalase.

Introduction: An enzyme is a biological catalyst made of protein.

Substrate concentration and yeast catalase
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